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Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats

Fatmah Ali Matough, Siti Balkis Budin, Zariyantey Abdul Hamid, Santhana Raj Louis, Nasar Alwahaibi, Jamaludin Mohamed.

Cited by (8)

Abstract
Hyperglycemia-induced oxidative stress in diabetic conditions promotes alterations of function and other properties of the erythrocyte membrane. In this study, we examined the effects of palm vitamin E (200 mg/kg) on oxidative stress and the physical and morphological characteristics of erythrocyte membranes in streptozotocin-induced diabetic rats. Forty male rats were randomly divided into 4 groups as follows: Group 1, control group; Group 2, normal plus palm vitamin E treatment; Group 3, untreated diabetic group; and Group 4, diabetic plus palm vitamin E treatment. After 4 weeks of treatments, we determined complete blood count, erythrocyte osmotic fragility, erythrocyte morphology, levels of erythrocyte malondialdehyde and protein carbonyl, levels of erythrocyte glutathione and oxidized glutathione, and total plasma antioxidant capacity. The results of osmotic fragility were significantly higher in the diabetic group compared to the control and diabetic with palm vitamin E. There were significant morphological changes in the erythrocytes of the diabetic group compared to the control group. We also found a significant decrease in the hemoglobin concentration, hematocrit percentage, and erythrocyte distribution width in the diabetic group. Erythrocyte malondialdehyde and protein carbonyl levels were significantly higher in the diabetic group compared to the control group. Decreased levels of reduced glutathione and increased level of oxidized glutathione were observed in the diabetic group compared to the control and diabetic with palm vitamin E groups. Plasma total antioxidant capacity was significantly lower in the diabetic group than in the control group, whereas plasma total antioxidants in the diabetic with palm vitamin E group were significantly increased compared to the untreated diabetic group. In conclusion, 4 weeks of daily supplementation with 200 mg/kg palm vitamin E could reduce the levels of oxidative stress markers by inhibiting lipid peroxidation and protein oxidation in streptozotocin-induced diabetic rats.

Key words: Diabetes mellitus; Erythrocyte membrane; Lipid peroxidation; Oxidative stress; Palm vitamin E


 
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How to Cite this Article
Pubmed Style

Matough FA, Budin SB, Hamid ZA, Louis SR, Alwahaibi N, Mohamed J. Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats. Oxid Antioxid Med Sci. 2012; 1(1): 59-68. doi:10.5455/oams.300412.or.006


Web Style

Matough FA, Budin SB, Hamid ZA, Louis SR, Alwahaibi N, Mohamed J. Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats. http://www.oamsjournal.com/?mno=14996 [Access: January 21, 2018]. doi:10.5455/oams.300412.or.006


AMA (American Medical Association) Style

Matough FA, Budin SB, Hamid ZA, Louis SR, Alwahaibi N, Mohamed J. Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats. Oxid Antioxid Med Sci. 2012; 1(1): 59-68. doi:10.5455/oams.300412.or.006



Vancouver/ICMJE Style

Matough FA, Budin SB, Hamid ZA, Louis SR, Alwahaibi N, Mohamed J. Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats. Oxid Antioxid Med Sci. (2012), [cited January 21, 2018]; 1(1): 59-68. doi:10.5455/oams.300412.or.006



Harvard Style

Matough, F. A., Budin, S. B., Hamid, Z. A., Louis, S. R., Alwahaibi, N. & Mohamed, J. (2012) Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats. Oxid Antioxid Med Sci, 1 (1), 59-68. doi:10.5455/oams.300412.or.006



Turabian Style

Matough, Fatmah Ali, Siti Balkis Budin, Zariyantey Abdul Hamid, Santhana Raj Louis, Nasar Alwahaibi, and Jamaludin Mohamed. 2012. Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats. Oxidants and Antioxidants in Medical Science, 1 (1), 59-68. doi:10.5455/oams.300412.or.006



Chicago Style

Matough, Fatmah Ali, Siti Balkis Budin, Zariyantey Abdul Hamid, Santhana Raj Louis, Nasar Alwahaibi, and Jamaludin Mohamed. "Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats." Oxidants and Antioxidants in Medical Science 1 (2012), 59-68. doi:10.5455/oams.300412.or.006



MLA (The Modern Language Association) Style

Matough, Fatmah Ali, Siti Balkis Budin, Zariyantey Abdul Hamid, Santhana Raj Louis, Nasar Alwahaibi, and Jamaludin Mohamed. "Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats." Oxidants and Antioxidants in Medical Science 1.1 (2012), 59-68. Print. doi:10.5455/oams.300412.or.006



APA (American Psychological Association) Style

Matough, F. A., Budin, S. B., Hamid, Z. A., Louis, S. R., Alwahaibi, N. & Mohamed, J. (2012) Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats. Oxidants and Antioxidants in Medical Science, 1 (1), 59-68. doi:10.5455/oams.300412.or.006





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