ISSN 2146-8389
 

Original Research (Original Article) Open Access


Determination of the antioxidant potential of goat sperm cells

Sudipta Saha, Sandhya Rekha Dungdung, Gopal Chandra Majumder.

Abstract
Objective: Reactive oxygen species (ROS) such as the superoxide anion, hydroxyl radical and hydrogen peroxide are highly toxic to cells. Cellular antioxidant enzymes and free radical scavengers normally protect a cell from toxic effects of ROS. However when generation of ROS overtakes the antioxidant defense then tissue damage is caused. The present study aims to find the ascorbic acid levels in sperm cells of different parts of the epididymis and the relation of level of ascorbic acid with motility status of cauda sperm cells considering goat sperm as a model system. The antioxidant potential of motile cauda sperm cells was also revealed.
Methods: Ascorbic acid assay and antioxidant potential assay was done to measure the levels of ascorbic acid and the levels of scavenging of free radicals. Sperm motility was determined by microscopic and spectrophotometric methods.
Results: From the results of the present study it was revealed that the level of ascorbic acid is significantly less in the sperm cells of the cauda epididymis. The level of total antioxidant potential of the sperm cells of the cauda epididymis was tested. The sperms were found to be having significant amount of antioxidants. No significant relation could be concluded regarding the levels of ascorbic acid in the sperms of the cauda epididymis and their motility status.
Conclusions: Since the sperms of the cauda epididymis are mostly motile, so, less ascorbic acid in cauda sperm may be due to the consumption of the antioxidant (ascorbic acid) by the sperm cells to scavenge the free radicals produced during motility. Reduction of ROS or free radicals seems to be necessary for sperm motility.

Key words: Ascorbic acid; Free radicals; Goat; Reactive oxygen species; Sperm motility


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Sudipta Saha
Articles by Sandhya Rekha Dungdung
Articles by Gopal Chandra Majumder
on Google
on Google Scholar
Article Statistics
 Viewed: 1223
Downloaded: 366
Cited: 0

REFERENCES
1. Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008; 4:89-96. [Pubmed]    [PMC Free Fulltext]   
2. Celep GS, Marotta F. Oxidants and antioxidants in health and disease. Oxid Antioxid Med Sci 2014; 3:5-8. [DOI via Crossref]   
3. Harman D. Free radical involvement in aging. Pathophysiology and therapeutic implications. Drug Aging 1993; 3:60-8. [DOI via Crossref]    [Pubmed]   
4. Fridovich I. Superoxide radical: an endogenous toxicant. Annu Pharmacol Toxicol 1983; 23:239-57. [DOI via Crossref]    [Pubmed]   
5. Walczak-Jedrzejowska R, Wolski JK, Slowikowska-Hilczer J. The role of oxidative stress and antioxidants in male fertility. Cent European J Urol. 2013; 66:60-7. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
6. Pahune PP, Choudhari AR, Muley PA. The total antioxidant power of semen and its correlation with the fertility potential of human male subjects. J Clin Diagn Res 2013; 7:991-5. [Pubmed]    [PMC Free Fulltext]   
7. El-Taieb MA, Herwig R, Nada EA, Greilberger J, Marberger M. Oxidative stress and epididymal sperm transport, motility and morphological defects. Eur J Obstet Gynecol Reprod Biol 2009; 144:S199-203. [DOI via Crossref]    [Pubmed]   
8. Bandyopadhyay U, Das D, Banerjee RK. Reactive oxygen species: oxidative damage and pathogenesis. Curr Sci 1999; 77:658-66.
9. Fridovich I. Biological effects of the superoxide radical. Arch Biochem Biophys 1986; 247:1-11. [DOI via Crossref]   
10. Meister A, Anderson ME. Glutathione. Ann Rev Biochem 1983; 52:711-60. [DOI via Crossref]    [Pubmed]   
11. Freeman BA, Carpo JD. Biology of disease: free radicals and tissue injury. Lab Invest 1982; 47:412-20. [Pubmed]   
12. Yamazaki I. Peroxidase. In: Hayaishi O (ed) Molecular Mechanism of O2 activation, Academic Press, New York, NY, pp. 535-558, 1974. [DOI via Crossref]   
13. Banerjee RK. Membrane peroxidises. Mol Cell Biochem 1988; 83:105-28. [DOI via Crossref]    [Pubmed]   
14. Deisseroth A, Doume AL. Catalase: physical and chemical properties, mechanism of catalysis, and physiological role. Physiol Rev 1970; 50:319-75. [Pubmed]   
15. Chen SJ, Allam JP, Duan YG, Haidl G. Influence of reactive oxygen species on human sperm functions and fertilizing capacity including therapeutical approaches. Arch Gynecol Obstet 2013; 288:191-9. [DOI via Crossref]    [Pubmed]   
16. Tvrda E, Knazicka Z, Bardos L, Massanyi P, Lukac N. Impact of oxidative stress on male fertility - a review. Acta Vet Hung 2011; 59:465-84. [DOI via Crossref]    [Pubmed]   
17. Chatterjee CC. Human Physiology. Medical Allied Agency, Calcutta, Volume 2, p 139, 1980.
18. Nelson DL, Cox MM. Lehninger Principles of Biochemistry. 3rd edition, Worth Publishers, New York, NY, p 743, 2000.
19. Salem MH, Kamel KI, Yousef MI, Hassan GA, Nouty FD. Protective role of ascorbic acid to enhance semen quality of rabbits treated with sublethal doses of aflatoxin B(1). Toxicology 2001; 162:209-18. [DOI via Crossref]   
20. Donnelly ET, McClure N, Lewis SE. Antioxidant supplementation in vitro does not improve human sperm motility. Fertil Steril 1999; 72:484-95. [DOI via Crossref]   
21. Verma A, Kanwar KC. Human sperm motility and lipid peroxidation in different ascorbic acid concentrations: an in vitro analysis. Andrologia 1998; 30:325-9. [DOI via Crossref]    [Pubmed]   
22. Hsu PC, Liu MY, Hsu CC, Chen LY, Guo YL. Toxicity in the rat sperm. Toxicology 1998; 128:169-79. [DOI via Crossref]   
23. Sanchez-Partida LG, Setchell BP, Maxwell WM. Epididymal compounds and antioxidants in diluents for the frozen storage of ram spermatozoa. Reprod Fertil Dev 1997; 9:689-96. [DOI via Crossref]    [Pubmed]   
24. O'Flaherty C, Beconi M, Beorlegui N. Effect of natural antioxidants, superoxide dismutase and hydrogen peroxide on capacitation of frozen-thawed bull spermatozoa. Andrologia 1997; 29:269-75. [DOI via Crossref]    [Pubmed]   
25. Ciereszko A, Dabrowski K. Sperm quality and ascorbic acid concentration in rainbow trout semen are affected by dietary vitamin C: an across-season study. Biol Reprod 1995; 52:982-8. [DOI via Crossref]   
26. Yousef MI, Abdallah GA, Kamel KI. Effect of ascorbic acid and Vitamin E supplementation on semen quality and biochemical parameters of male rabbits. Anim Reprod Sci 2003; 76:99-111. [DOI via Crossref]   
27. Fanaei H, Khayat S, Halvaei I, Ramezani V, Azizi Y, Kasaeian A, Mardaneh, J, Parvizi MR, Akrami M. Effects of ascorbic acid on sperm motility, viability, acrosome reaction and DNA integrity in teratozoospermic samples. Iran J Reprod Med 2014; 12:103-10. [Pubmed]    [PMC Free Fulltext]   
28. Nadjarzadeh A, Mehrsai A, Mostafavi E, Gohari MR, Shidfar F. The association between dietary antioxidant intake and semen quality in infertile men. Med J Islam Repub Iran 2013; 27:204-9. [Pubmed]    [PMC Free Fulltext]   
29. Foote RH, Brockett CC, Kaproth MT. Motility and fertility of bull sperm in whole milk extender containing antioxidants. Anim Reprod Sci 2002; 71:13-23. [DOI via Crossref]   
30. Chitra KC, Rao KR, Mathur PP. Effect of experimental varicocele on structure and function of epididymis in adolescent rats: a histological and biochemical study. Asian J Androl 2003; 5:203-8. [Pubmed]   
31. Gangadharan B, Murugan MA, Mathur PP. Effect of methoxychlor on antioxidant system of goat epididymal sperm in vitro. Asian J Androl 2001; 3:285-8. [Pubmed]   
32. Majumder GC, Chakrabarti CK. A simple spectrophotometric method of assay of forward motility of goat spermatozoa. J Reprod Fertil 1984; 70:235-41. [DOI via Crossref]   
33. Mandal M, Banerjee S, Majumder GC. Stimulation of forward motility of goat cauda epididymal spermatozoa by a serum glycoprotein factor. Biol Reprod 1989; 41:983-9. [DOI via Crossref]    [Pubmed]   
34. Roy N, Majumder GC. A simple quantitative method for the estimation of free ecto-sulfhydryl groups of spermatozoa. Exp Cell Res 1986; 164:415-25. [DOI via Crossref]   
35. Saha S, Das S, Bhoumik A, Ghosh P, Majumder GC, Dungdung SR. Identification of a novel sperm motility stimulating protein from caprine serum: its characterization and functional significance. Fertil Steril 2013; 100:269-79. [DOI via Crossref]    [Pubmed]   
36. Mandal M, Banerjee S. Majumder GC. Stimulation of forward motility of goat cauda epididymis spermatozoa by a sperm glycoprotein factor. Biol Reprod 1989; 41:983-9. [DOI via Crossref]   
37. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265-75. [Pubmed]   
38. Kyaw A. A simple colorimetric method for ascorbic acid determination in blood plasma. Clin Chim Acta 1978; 86:153-7. [DOI via Crossref]   
39. Babbs CF, Steiner MG. Detection and quantitation of hydroxy radical using dimethyl sulfoxide as molecular probe. Methods Enzymol 1990; 186:137-47. [DOI via Crossref]   
40. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. World J Mens Health 2014; 32:1-17. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   

How to Cite this Article
Pubmed Style

Saha S, Dungdung SR, Majumder GC. Determination of the antioxidant potential of goat sperm cells. Oxid Antioxid Med Sci. 2014; 3(3): 195-200. doi:10.5455/oams.290914.or.075


Web Style

Saha S, Dungdung SR, Majumder GC. Determination of the antioxidant potential of goat sperm cells. http://www.oamsjournal.com/?mno=165028 [Access: January 23, 2018]. doi:10.5455/oams.290914.or.075


AMA (American Medical Association) Style

Saha S, Dungdung SR, Majumder GC. Determination of the antioxidant potential of goat sperm cells. Oxid Antioxid Med Sci. 2014; 3(3): 195-200. doi:10.5455/oams.290914.or.075



Vancouver/ICMJE Style

Saha S, Dungdung SR, Majumder GC. Determination of the antioxidant potential of goat sperm cells. Oxid Antioxid Med Sci. (2014), [cited January 23, 2018]; 3(3): 195-200. doi:10.5455/oams.290914.or.075



Harvard Style

Saha, S., Dungdung, S. R. & Majumder, G. C. (2014) Determination of the antioxidant potential of goat sperm cells. Oxid Antioxid Med Sci, 3 (3), 195-200. doi:10.5455/oams.290914.or.075



Turabian Style

Saha, Sudipta, Sandhya Rekha Dungdung, and Gopal Chandra Majumder. 2014. Determination of the antioxidant potential of goat sperm cells. Oxidants and Antioxidants in Medical Science, 3 (3), 195-200. doi:10.5455/oams.290914.or.075



Chicago Style

Saha, Sudipta, Sandhya Rekha Dungdung, and Gopal Chandra Majumder. "Determination of the antioxidant potential of goat sperm cells." Oxidants and Antioxidants in Medical Science 3 (2014), 195-200. doi:10.5455/oams.290914.or.075



MLA (The Modern Language Association) Style

Saha, Sudipta, Sandhya Rekha Dungdung, and Gopal Chandra Majumder. "Determination of the antioxidant potential of goat sperm cells." Oxidants and Antioxidants in Medical Science 3.3 (2014), 195-200. Print. doi:10.5455/oams.290914.or.075



APA (American Psychological Association) Style

Saha, S., Dungdung, S. R. & Majumder, G. C. (2014) Determination of the antioxidant potential of goat sperm cells. Oxidants and Antioxidants in Medical Science, 3 (3), 195-200. doi:10.5455/oams.290914.or.075





Most Viewed Articles
  • Low intensity radiofrequency radiation: a new oxidant for living cells
    Igor Yakymenko, Evgeniy Sidorik, Diane Henshel, Sergiy Kyrylenko
    Oxid Antioxid Med Sci. 2014; 3(1): 1-3
    » Abstract & References » doi: 10.5455/oams.240314.ed.002

  • Oxidants and antioxidants: friends or foes?
    Sukru Oter, Si Jin, Luca Cucullo, H.J. Damien Dorman
    Oxid Antioxid Med Sci. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/oams.080612.ed.001

  • Effects of free radicals and antioxidants on exercise performance
    Sikiru Lamina, Charles I. Ezema, Anele I. Theresa, Ezugwu U. Anthonia
    Oxid Antioxid Med Sci. 2013; 2(2): 83-91
    » Abstract & References » doi: 10.5455/oams.010413.rv.005

  • Effects of silymarin, N-acetylcysteine and selenium in the treatment of papulopustular acne
    Haidar Hamid Al-Anbari, Ahmed Salih Sahib, Ahmed R. Abu Raghif
    Oxid Antioxid Med Sci. 2012; 1(3): 201-207
    » Abstract & References » doi: 10.5455/oams.290912.or.019

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005

  • Is it oxidative or free radical stress and why does it matter?
    Boguslaw Lipinski
    Oxid Antioxid Med Sci. 2012; 1(1): 5-9
    » Abstract & References » doi: 10.5455/oams.130312.rv.001

  • Chemical properties of Monodora myristica and its protective potentials against free radicals in vitro
    Ochuko L. Erukainure, Oluwatoyin V. Oke, Folashade O. Owolabi, Funmi O. Kayode, Emmanuel E. Umanhonlen, Muhammad Aliyu
    Oxid Antioxid Med Sci. 2012; 1(2): 127-132
    » Abstract & References » doi: 10.5455/oams.080712.or.009

  • Cytotoxic and proapoptotic activities of gallic acid to human oral cancer HSC-2 cells
    Alyssa G. Schuck, Jeffrey H. Weisburg, Hannah Esan, Esther F. Robin, Ayelet R. Bersson, Jordana R. Weitschner, Tova Lahasky, Harriet L. Zuckerbraun, Harvey Babich
    Oxid Antioxid Med Sci. 2013; 2(4): 265-274
    » Abstract & References » doi: 10.5455/oams.220713.or.051

  • Phytochemical and in vitro antioxidant properties of the methanolic extract of fruits of Blighia sapida, Vitellaria paradoxa and Vitex doniana
    Rabiat U. Hamzah, Evans C. Egwim, Adamu Y. Kabiru, Mary B. Muazu
    Oxid Antioxid Med Sci. 2013; 2(3): 217-223
    » Abstract & References » doi: 10.5455/oams.090513.or.043

  • Most Downloaded
  • Low intensity radiofrequency radiation: a new oxidant for living cells
    Igor Yakymenko, Evgeniy Sidorik, Diane Henshel, Sergiy Kyrylenko
    Oxid Antioxid Med Sci. 2014; 3(1): 1-3
    » Abstract & References » doi: 10.5455/oams.240314.ed.002

  • Oxidants and antioxidants: friends or foes?
    Sukru Oter, Si Jin, Luca Cucullo, H.J. Damien Dorman
    Oxid Antioxid Med Sci. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/oams.080612.ed.001

  • The antioxidant capacity and immunomodulatory activity of stingless bee honeys proceeding from Costa Rica
    Gabriel Zamora, Kees Beukelman, Bert van den Berg, Maria Laura Arias, Eduardo Umana, Ingrid Aguilar, Linda Quarles van Ufford, Edwin van den Worm, Natalia Fallas, Rebeca Solorzano
    Oxid Antioxid Med Sci. 2015; 4(1): 49-55
    » Abstract & References » doi: 10.5455/oams.180415.or.084

  • Is it oxidative or free radical stress and why does it matter?
    Boguslaw Lipinski
    Oxid Antioxid Med Sci. 2012; 1(1): 5-9
    » Abstract & References » doi: 10.5455/oams.130312.rv.001

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038

  • Effects of free radicals and antioxidants on exercise performance
    Sikiru Lamina, Charles I. Ezema, Anele I. Theresa, Ezugwu U. Anthonia
    Oxid Antioxid Med Sci. 2013; 2(2): 83-91
    » Abstract & References » doi: 10.5455/oams.010413.rv.005

  • Protective properties of complex of quercetin, selenium, catechins and curcumin against DNA damage
    Jana Kadrabova, Marica Krajcovicova-Kudlackova, Alexander Madaric, Martina Valachovicova, Csilla Mislanova, Maria Korenovska
    Oxid Antioxid Med Sci. 2012; 1(3): 179-184
    » Abstract & References » doi: 10.5455/oams.180912.or.018

  • Airway antioxidant capacity and pH in chronic obstructive pulmonary disease
    Wei Lee, Hsien Loo, Paul S. Thomas
    Oxid Antioxid Med Sci. 2012; 1(3): 153-160
    » Abstract & References » doi: 10.5455/oams.300812.or.016

  • Heparin or EDTA; anticoagulant of choice in free radical estimation?
    Kuldeep Mohanty, Swetasmita Mishra, Jhumur Pani, Tarannum Hasan, Abhishek Purohit, Subhadra Sharma, Rima Dada
    Oxid Antioxid Med Sci. 2012; 1(1): 21-24
    » Abstract & References » doi: 10.5455/oams.130512.br.001

  • Most Cited Articles
  • Chemical properties of Monodora myristica and its protective potentials against free radicals in vitro
    Ochuko L. Erukainure, Oluwatoyin V. Oke, Folashade O. Owolabi, Funmi O. Kayode, Emmanuel E. Umanhonlen, Muhammad Aliyu
    Oxid Antioxid Med Sci. 2012; 1(2): 127-132
    » Abstract & References » doi: 10.5455/oams.080712.or.009
    Cited : 11 times [Click to see citing articles]

  • Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines
    Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Yellianty Yellianty
    Oxid Antioxid Med Sci. 2013; 2(2): 137-142
    » Abstract & References » doi: 10.5455/oams.100413.or.038
    Cited : 10 times [Click to see citing articles]

  • Silymarin and naringenin protects nicotine induced oxidative stress in young rats
    Anshu Jain, Nidhi Dwivedi, Rakesh Bhargava, Swaran J.S. Flora
    Oxid Antioxid Med Sci. 2012; 1(1): 41-49
    » Abstract & References » doi: 10.5455/oams.130412.or.004
    Cited : 9 times [Click to see citing articles]

  • Role of free radicals and antioxidants in gynecological cancers: current status and future prospects
    Lokanatha Valluru, Subramanyam Dasari, Rajendra Wudayagiri
    Oxid Antioxid Med Sci. 2014; 3(1): 15-26
    » Abstract & References » doi: 10.5455/oams.201113.rv.011
    Cited : 9 times [Click to see citing articles]

  • Palm vitamin E reduces oxidative stress, and physical and morphological alterations of erythrocyte membranes in streptozotocin-induced diabetic rats
    Fatmah Ali Matough, Siti Balkis Budin, Zariyantey Abdul Hamid, Santhana Raj Louis, Nasar Alwahaibi, Jamaludin Mohamed
    Oxid Antioxid Med Sci. 2012; 1(1): 59-68
    » Abstract & References » doi: 10.5455/oams.300412.or.006
    Cited : 8 times [Click to see citing articles]

  • Phytochemical and in vitro antioxidant properties of the methanolic extract of fruits of Blighia sapida, Vitellaria paradoxa and Vitex doniana
    Rabiat U. Hamzah, Evans C. Egwim, Adamu Y. Kabiru, Mary B. Muazu
    Oxid Antioxid Med Sci. 2013; 2(3): 217-223
    » Abstract & References » doi: 10.5455/oams.090513.or.043
    Cited : 8 times [Click to see citing articles]

  • Peroxidative index as novel marker of hydrogen peroxide involvement in lipid peroxidation from coal dust exposure
    Nia Kania, Bambang Setiawan, Edi Widjajanto, Nurdiana Nurdiana, M. Aris Widodo, H.M.S. Chandra Kusuma
    Oxid Antioxid Med Sci. 2012; 1(3): 209-215
    » Abstract & References » doi: 10.5455/oams.031012.or.020
    Cited : 7 times [Click to see citing articles]

  • Heparin or EDTA; anticoagulant of choice in free radical estimation?
    Kuldeep Mohanty, Swetasmita Mishra, Jhumur Pani, Tarannum Hasan, Abhishek Purohit, Subhadra Sharma, Rima Dada
    Oxid Antioxid Med Sci. 2012; 1(1): 21-24
    » Abstract & References » doi: 10.5455/oams.130512.br.001
    Cited : 6 times [Click to see citing articles]

  • Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7
    Mohankumar Thenmozhi, Krishnan Kannabiran
    Oxid Antioxid Med Sci. 2012; 1(1): 51-57
    » Abstract & References » doi: 10.5455/oams.270412.or.005
    Cited : 6 times [Click to see citing articles]

  • Cytotoxic and proapoptotic activities of gallic acid to human oral cancer HSC-2 cells
    Alyssa G. Schuck, Jeffrey H. Weisburg, Hannah Esan, Esther F. Robin, Ayelet R. Bersson, Jordana R. Weitschner, Tova Lahasky, Harriet L. Zuckerbraun, Harvey Babich
    Oxid Antioxid Med Sci. 2013; 2(4): 265-274
    » Abstract & References » doi: 10.5455/oams.220713.or.051
    Cited : 6 times [Click to see citing articles]