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Antioxidant and antimicrobial profile of a secondary metabolite of <i>Pseudomonas aeruginosa</i>: ESR and spectrophotometric method

E. Madhava Charyulu, Japyesan Subramanian, Gnanamani Arumugam.

Abstract
Marine <i>Pseudomonas</i> is a potential provider of novel bioactive metabolites and currently emerged as an important source for natural products with unique chemical diversity. The aim of the present study was to evaluate antimicrobial and antioxidant potential of partially purified fraction III extracted as a secondary metabolite from <i>Pseudomonas aeruginosa</i> MTCC 5210 isolated from marine sediments of coastal Tamil Nadu, India. In the present study, the antioxidant property of fraction III was evaluated using spectrophotometric and alectron spin resonance (ESR) techniques. The results of these studies revealed, fraction III quenches 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH●), nitric oxide radical and superoxide radical at appreciable levels. The ESR measurements show the reduction in peak intensities of DPPH● and nitric oxide radical after the addition of fraction III. It was also observed that quenching and reduction in peak intensities of standard free radicals studied are in proportion to the concentrations of fraction III studied. The antimicrobial activity of fraction III was evaluated using antibiotic resistant microbes such as methicillin resistant <i>Staphylococcus aureus</i> (MRSA). Since there is a constant demand for the natural antioxidants, the results of our present study suggest, that fraction III may act as a potential antioxidant and antibacterial compound.

Key words: Antibacterial activity; DPPH●; ESR; Nitric oxide; <i>Pseudomonas aeruginosa</i>; Superoxide


 
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REFERENCES
1.Kushi LH, Byers T, Doyle C, Bandera EV, McCullough M, McTiernan A, Gansler T, Andrews KS, Thun MJ; American Cancer Society 2006 Nutrition and Physical Activity Guidelines Advisory Committee. American Cancer Society Guidelines on Nutrition and Physical Activity for cancer prevention: reducing the risk of cancer with healthy food choices and physical activity. CA Cancer J Clin 2006; 56:254-81.

http://dx.doi.org/10.3322/canjclin.56.5.254

[Pubmed]   

2.Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001; 54:176-86.

http://dx.doi.org/10.1136/jcp.54.3.176

[
Pubmed]    [PMC Free Fulltext]   

3.Moure A, Cruz JM, Franco D, Domnguez JM, Sineiro J, Domnguez H, Jose Nunez M, Parajo JC. Natural antioxidants from residual sources. Food Chem 2001; 72:145-71.

http://dx.doi.org/10.1016/S0308-8146(00)00223-5


4.Wang W, Wu N, Zu YG, Fu YJ. Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components. Food Chem 2008; 108:1019-22.

http://dx.doi.org/10.1016/j.foodchem.2007.11.046


5.Sherwin FR. Antioxidants. In: Branen AL, Davidson PM, Salminen S (eds) Food Additives, Marcel Dekker, New York, NY, USA, pp 139-193, 1990.


6.Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 1995; 28:25-30.

http://dx.doi.org/10.1016/S0023-6438(95)80008-5


7.Yen GC, Chang YC. Production of antioxidant from Aspergillus candidus broth filtrate by fermentor. Process Biochem 2003; 38:1425-30.

http://dx.doi.org/10.1016/S0032-9592(03)00028-1


8.Smith JL, Alford JA. Presence of antioxidant materials in bacteria. Lipids 1970; 5:795-9.

http://dx.doi.org/10.1007/BF02531970

[
Pubmed]   

9.Hayashi KI, Suzuki K, Kawaguchi M, Nakajima T, Suzuki T, Numata M, Nakamura T. Isolation of an antioxidant from Penicillium roquefortii IFO 5956. Biosci Biotech Biochem 1995; 59:319-20.

http://dx.doi.org/10.1271/bbb.59.319

[
Pubmed]   

10.Hirota A, Morimitsu Y, Hojo H. New antioxidative indophenol-reducing phenol compounds isolated from the Mortierella sp. fungus. Biosci Biotech Biochem 1997; 61:647-50.

http://dx.doi.org/10.1271/bbb.61.647


11.Charyulu EM, Sekaran G, Rajakumar GS, Gnanamani A. Antimicrobial activity of secondary metabolite from marine isolate, Pseudomonas sp against Gram positive and negative bacteria including MRSA. Ind J Exp Biol 2009; 47:964-8.

[
Pubmed]   

12.Blois MS. Antioxidant determination by the use of a stable free radical. Nature 1958; 181:1199-200.

http://dx.doi.org/10.1038/1811199a0


13.Marcocci L, Maguire JJ, Droylefaix MT, Packer L. The nitric oxidescavenging properties of Ginkgo biloba extract EGb 761. Biochem Biophys Res Comm 1994; 201:748-55.

http://dx.doi.org/10.1006/bbrc.1994.1764

[
Pubmed]   

14.Fontana M, Mosca L, Rosei MA. Interaction of enkephalins with oxyradicals. Biochem Pharmacol 2001; 61:1253-57.

http://dx.doi.org/10.1016/S0006-2952(01)00565-2

[
Pubmed]   

15.Sripriya G, Chandrasekharan K, Murty VS, Chandra TS. ESR spectroscopic studies on free radical quenching action of finger millet (Eleusine coracana). Food Chem 1996; 57:537-40.

http://dx.doi.org/10.1016/S0308-8146(96)00187-2


16.Venkataraman S, Martin SM, Schafer FQ, Buettner GR. Detailed methods for the quantification of nitric oxide in aqueous solutions using either an oxygen monitor or EPR. Free Radic Biol Med 2000; 29:580-5.

http://dx.doi.org/10.1016/S0891-5849(00)00404-4

[
Pubmed]   

17.Perez C, Paul M, Bazerque P. An antibiotic assay by the agar well diffusion method. Acta Biol Med Exp 1990; 15:113-5.


18.Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother 2001; 48:5-16.

http://dx.doi.org/10.1093/jac/48.suppl_1.5

[
Pubmed]   

19.Sanchez-Moreno C. Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int 2002; 121-37.


20.Halliwell B, Gutteridge JMC. The definition and measurement of antioxidants in biological systems. Free Radic Biol Med 1995; 18:125-6.

http://dx.doi.org/10.1016/0891-5849(95)91457-3

[
Pubmed]   

21.Slater TF. Free-radical mechanisms in tissue injury. Biochem J 1984; 222:1-15.

[
Pubmed]    [PMC Free Fulltext]   

22.Jovanovic SV, Simic MG. Antioxidants in nutrition. Ann NY Acad Sci 2000; 899:326-34.

http://dx.doi.org/10.1111/j.1749-6632.2000.tb06197.x

[
Pubmed]   

23.Wickens AP. Ageing and the free radical theory. Respir Physiol 2001; 128:379-91.

http://dx.doi.org/10.1016/S0034-5687(01)00313-9

[
Pubmed]   

24.Barreira JCM, Ferreira ICFR, Oliveira MBPP, Pereira JA. Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem 2008; 107:1106-13.

http://dx.doi.org/10.1016/j.foodchem.2007.09.030


25.Devi KP, Suganthy N, Kesika P, Pandian SK. Bioprotective properties of seaweeds: in vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content. BMC Complement Altern Med 2008; 8:38.

http://dx.doi.org/10.1186/1472-6882-8-38

[
Pubmed]    [PMC Free Fulltext]   

26.Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Altern Med 2008; 8:63-73.

http://dx.doi.org/10.1186/1472-6882-8-63

[
Pubmed]    [PMC Free Fulltext]   

27.Meir S, Kanner J, Akiri B, Philosoph-Hadas S. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 1995; 43:1813-9.

http://dx.doi.org/10.1021/jf00055a012


28.Gordon MH. The mechanism of antioxidant action in vitro. In: Hudson BJF (ed) Food Antioxidants, Elsevier, London, UK, pp. 1–18, 1990.

http://dx.doi.org/10.1007/978-94-009-0753-9_1


29.Huang DJ, Ou BX, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005; 53:1841-56.

http://dx.doi.org/10.1021/jf030723c

[
Pubmed]   

30.Halliwell B. Antioxidants: the basics-what they are and how to evaluate them. Adv Pharmacol 1996; 38:3-20.

http://dx.doi.org/10.1016/S1054-3589(08)60976-X

[
Pubmed]   

31.Hu C, Kitts DD. Studies on the antioxidant activity of Echinacea root extract. J Agric Food Chem 2000; 48:1466-72.

http://dx.doi.org/10.1021/jf990677+

[
Pubmed]   

32.Yu L, Haley S, Perret J, Harris M, Wilson J, Aian M. Free radical scavenging properties of wheat extracts. J Agric Food Chem 2002; 50:1619-24.

http://dx.doi.org/10.1021/jf010964p

[
Pubmed]   

33.Halliwell B, Aeschbach R, Loliger J, Aruoma OI. The characterization of antioxidants. Food Chem Toxicol 1995; 33:601-17.

http://dx.doi.org/10.1016/0278-6915(95)00024-V

[
Pubmed]   

34.Fu HY, Shieh DE, Ho CT. Antioxidant and free radical scavenging activities of edible mushrooms. J Food Lipids 2002; 9:35-43.

http://dx.doi.org/10.1111/j.1745-4522.2002.tb00206.x


35.Stevanato R, Fabris S, Momo F. Enzymatic method for the determination of total phenolic content in tea and wine. J Agric Food Chem 2004; 52:6287-93.

http://dx.doi.org/10.1021/jf049898s

[
Pubmed]   

36.Yu L. Free radical scavenging properties of conjugated linoleic acids. J Agric Food Chem 2001; 49:3452-56.

http://dx.doi.org/10.1021/jf010172v

[
Pubmed]   

37.Moncada S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 1991; 43:109-42.

[
Pubmed]   

38.Lekameera R, Vijayabaskar P, Somasundaram ST. Evaluating antioxidant property of brown ALGA Colpomenia sinuosa (DERB. ET SOL). Afr J Food Sci 2008; 2:126-30.


39.Sasaki S, Miura T, Nishikawa S, Yamada K, Hirasue M, Nakane A. Protective role of nitric oxide in Staphylococcus aureus infection in mice. Infect Immun 1998; 66:1017-22.

[
Pubmed]    [PMC Free Fulltext]   

40.Ozen T, Kinalioglu K. Determination of antioxidant activity of various extracts of Parmelia saxatilis. Biologia 2008; 63:211-6.

http://dx.doi.org/10.2478/s11756-008-0047-6


41.Cotelle N, Bernier JL, Catteau JP, Pommery J, Wallet JC, Gaydou EM. Antioxidant properties of hydroxy-flavones. Free Radic Biol Med 1996; 20:35-43.

http://dx.doi.org/10.1016/0891-5849(95)02014-4

[
Pubmed]   

42.Meyer AS, Isaksen A. Application of enzymes as food antioxidants. Trends Food Sci Tech 1995; 6:300-4.

http://dx.doi.org/10.1016/S0924-2244(00)89140-2

[
Pubmed]   

43.Singh N, Rajini PS. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 2004; 85:611-6.

http://dx.doi.org/10.1016/j.foodchem.2003.07.003


44.Kumaran A, Karunakaran RJ. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem 2006; 97:109-14.

http://dx.doi.org/10.1016/j.foodchem.2005.03.032


45.Ak T, Gulcin I. Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact 2008; 174:27-37.

http://dx.doi.org/10.1016/j.cbi.2008.05.003

[
Pubmed]   

How to Cite this Article
Pubmed Style

Charyulu EM, Subramanian J, Arumugam G. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxid Antioxid Med Sci. 2012; 1(2): 119-126. doi:10.5455/oams.200812.or.013


Web Style

Charyulu EM, Subramanian J, Arumugam G. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. http://www.oamsjournal.com/?mno=22582 [Access: January 21, 2018]. doi:10.5455/oams.200812.or.013


AMA (American Medical Association) Style

Charyulu EM, Subramanian J, Arumugam G. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxid Antioxid Med Sci. 2012; 1(2): 119-126. doi:10.5455/oams.200812.or.013



Vancouver/ICMJE Style

Charyulu EM, Subramanian J, Arumugam G. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxid Antioxid Med Sci. (2012), [cited January 21, 2018]; 1(2): 119-126. doi:10.5455/oams.200812.or.013



Harvard Style

Charyulu, E. M., Subramanian, J. & Arumugam, G. (2012) Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxid Antioxid Med Sci, 1 (2), 119-126. doi:10.5455/oams.200812.or.013



Turabian Style

Charyulu, E. Madhava, Japyesan Subramanian, and Gnanamani Arumugam. 2012. Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxidants and Antioxidants in Medical Science, 1 (2), 119-126. doi:10.5455/oams.200812.or.013



Chicago Style

Charyulu, E. Madhava, Japyesan Subramanian, and Gnanamani Arumugam. "Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method." Oxidants and Antioxidants in Medical Science 1 (2012), 119-126. doi:10.5455/oams.200812.or.013



MLA (The Modern Language Association) Style

Charyulu, E. Madhava, Japyesan Subramanian, and Gnanamani Arumugam. "Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method." Oxidants and Antioxidants in Medical Science 1.2 (2012), 119-126. Print. doi:10.5455/oams.200812.or.013



APA (American Psychological Association) Style

Charyulu, E. M., Subramanian, J. & Arumugam, G. (2012) Antioxidant and antimicrobial profile of a secondary metabolite of Pseudomonas aeruginosa: ESR and spectrophotometric method. Oxidants and Antioxidants in Medical Science, 1 (2), 119-126. doi:10.5455/oams.200812.or.013





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