ISSN 2146-8389
 

Original Research (Original Article) Open Access


Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma

Achmad Rofi'i, Fatchiyah Fatchiyah, Pudji Rahayu, Ruslan Muhyi, Sutiman Bambang Sumitro.

Cited by (1)

Abstract
This study aimed to evaluate the level of reactive oxygen species, oxidative stress, and NF-κB and p53 in undifferentiated nasopharyngeal carcinoma. Twenty-four nasopharyngeal carcinoma patients and ten normal subjects were involved in order to compare the level of malondialdehyde (MDA), hydrogen peroxide (H2O2), and peroxidative index (PI). The level of reactive oxygen species (ROS), expression of NF-κB and p53 from a biopsy specimen of nasopharyngeal carcinoma tissue (histologically confirmed to be undifferentiated WHO III) were also compared with normal nasopharyngeal tissue. The Student t-test was used to analyze the different level of MDA, H2O2 and PI. Analysis of MDA level and H2O2 was done by colorimetric method. Levels of ROS, NF-κB, and p53 were analyzed using laser scanning confocal microscopy. MDA and H2O2 levels as well as PI of nasopharyngeal carcinoma patients were significantly higher compared to control. The levels of ROS and expression of NF-κB and p53 were higher in nasopharyngeal carcinoma tissue than those in normal nasopharyngeal tissue. We conclude that the tissue of nasopharyngeal carcinoma is one source of ROS and oxidative stress in nasopharyngeal carcinoma. NF-κB and p53 levels in nasopharyngeal carcinoma tissue may contribute to oxidative stress in undifferentiated nasopharyngeal carcinoma.

Key words: Lipid peroxidation; NF-kB; p53; Reactive oxygen species


 
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This Article Cited By the following articles

PARP-1 expression against Epstein-Barr virus LMP-1 and BZLF-1 in undifferentiated nasopharyngeal carcinoma
Journal of Experimental and Integrative Medicine 2013; 3(4): 299.

1
 

How to Cite this Article
Pubmed Style

Rofi\i A, Fatchiyah F, Rahayu P, Muhyi R, Sumitro B. Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma. Oxid Antioxid Med Sci. 2013; 2(2): 143-147. doi:10.5455/oams.300313.or.033


Web Style

Rofi\i A, Fatchiyah F, Rahayu P, Muhyi R, Sumitro B. Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma. http://www.oamsjournal.com/?mno=32893 [Access: January 23, 2018]. doi:10.5455/oams.300313.or.033


AMA (American Medical Association) Style

Rofi\i A, Fatchiyah F, Rahayu P, Muhyi R, Sumitro B. Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma. Oxid Antioxid Med Sci. 2013; 2(2): 143-147. doi:10.5455/oams.300313.or.033



Vancouver/ICMJE Style

Rofi\i A, Fatchiyah F, Rahayu P, Muhyi R, Sumitro B. Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma. Oxid Antioxid Med Sci. (2013), [cited January 23, 2018]; 2(2): 143-147. doi:10.5455/oams.300313.or.033



Harvard Style

Rofi\i, A., Fatchiyah, F., Rahayu, P., Muhyi, R. & Sumitro, . B. (2013) Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma. Oxid Antioxid Med Sci, 2 (2), 143-147. doi:10.5455/oams.300313.or.033



Turabian Style

Rofi\i, Achmad, Fatchiyah Fatchiyah, Pudji Rahayu, Ruslan Muhyi, and Sutiman Bambang Sumitro. 2013. Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma. Oxidants and Antioxidants in Medical Science, 2 (2), 143-147. doi:10.5455/oams.300313.or.033



Chicago Style

Rofi\i, Achmad, Fatchiyah Fatchiyah, Pudji Rahayu, Ruslan Muhyi, and Sutiman Bambang Sumitro. "Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma." Oxidants and Antioxidants in Medical Science 2 (2013), 143-147. doi:10.5455/oams.300313.or.033



MLA (The Modern Language Association) Style

Rofi\i, Achmad, Fatchiyah Fatchiyah, Pudji Rahayu, Ruslan Muhyi, and Sutiman Bambang Sumitro. "Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma." Oxidants and Antioxidants in Medical Science 2.2 (2013), 143-147. Print. doi:10.5455/oams.300313.or.033



APA (American Psychological Association) Style

Rofi\i, A., Fatchiyah, F., Rahayu, P., Muhyi, R. & Sumitro, . B. (2013) Reactive oxygen species, NF-kB, and p53 levels in tissue of undifferentiated nasopharyngeal carcinoma. Oxidants and Antioxidants in Medical Science, 2 (2), 143-147. doi:10.5455/oams.300313.or.033





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